Originally Posted by
watercooled
Let me just re-iterate what FatalSaviour said, RAID is not backup! It's designed for redundancy and so can be useful for servers which need to have as little downtime as possible. RAID covers a single problem i.e. single physical HDD failure, not the countless other problems which can destroy data, for example, accidental deletion, malware, PSU failure, power cuts, and so on. I also strongly recommend you add the 250GB drive to your offline backup plan - you do backup right? Keeping it in the PC as a backup drive is also bad practice, a good deal of the problems listed above would still affect it so it's not really an adequate backup.
In answer to your question though, yes it's possible, but I really don't see the point in a PC. I'd rather either have the extra space or just take it out of the PC. Also if you get a 'green' drive with a lower spindle speed, your existing 250GB drive might be faster for an OS drive.
Edit: Oh and using RAID0 will not give you a 2.25TB drive. You will have to create a 250GB partition on the 2TB drive then RAID0 them together to create a 500GB volume, then another 1.75TB of un-raided space on the 2TB drive. JBOD may suit your needs better if you just want to create a big volume across both drives.